BioColl® Separating Solution, 1 litre

BioColl® separation solution (density 1.077 g/ml, isotonic).

BioColl® separation solutions contain polysucrose, a polymer with a molecular weight of about 400,000 Daltons. This hydrophilic polymer can be used to prepare aqueous solutions up to a density of about 1.2 g/ml. For optimum values in terms of pH and osmolality, BioColl® must be mixed with an acid, preferably amidotrizoic acid (ATS), and caustic soda.

Which density of BioColl® for which cell fraction?
An overview:

Platelets: density 1.040 - 1.060 g/ml

Monocytes: density 1.059 - 1.068 g/ml

Lymphocytes: density 1.066 - 1.077 g/ml

Basophils: density 1.075 - 1.081 g/ml

Neutrophils: density 1.080 - 1.099 g/ml

Eosinophils: density 1.088 - 1.096 g/ml

Erythroctyes: density 1.090 - 1.110 g/ml

 

 

BioColl® is a registered trademark of Bio&SELL GmbH.

Storage temp.: +2°C to RT