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BioColl® separating solution density 1.077 g/ml, with HEPES, 500 ml

BioColl® separating solution density 1.077 g/ml, with HEPES, 500 ml
€79.90 *
Content: 500 Milliliter

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Delivery time approx. 5 working days

  • BS.L.6715
BioColl® separation solution (density 1.077 g/ml, isotonic) with 10mM HEPES. BioColl®... more
Product information

BioColl® separation solution (density 1.077 g/ml, isotonic) with 10mM HEPES.

BioColl® separation solutions contain polysucrose, a polymer with a molecular weight of about 400,000 daltons. With this hydrophilic polymer, aqueous solutions up to a density of about 1.2 g/ml can be prepared. Depending on the target fraction, the appropriate density of the BioColl solution is selected (see list). For optimal values regarding pH and osmolality, BioColl® must be mixed with an acid, preferably amidotrizoic acid (ATS), and caustic soda.

Which density of BioColl® for which cell fraction?
An overview:

Platelets: density 1.040 - 1.060 g/ml

Monocytes: density 1.059 - 1.068 g/ml

Lymphocytes: density 1.066 - 1.077 g/ml

Basophils: density 1.075 - 1.081 g/ml

Neutrophils: density 1.080 - 1.099 g/ml

Eosinophils: density 1.088 - 1.096 g/ml

Erythroctyes: density 1.090 - 1.110 g/ml

 

 

BioColl® is a registered trademark of Bio&SELL GmbH.

Storage temp.: +2°C to RT

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Technical data


Kategorie:
Zentrifugationsmedium

Dichte: 1,077 g/ml

Puffersubstanz: 10 mM HEPES

Spezifizierung: Zur Lymphozyten-Isolierung

Form: Flüssigkeit (durchsichtig)

Volumen: 500 ml

Lagerung: +2°C bis RT

Steril filtriert: Ja

Zolltarifnummer: 38229000

FAQ


1. Welche Dichte der BioColl®-Trennlösung brauche ich für welche Zellfraktion?

Thrombozyten: Dichte 1,040 - 1,060 g/ml

Monozyten: Dichte 1,059 - 1,068 g/ml

Lymphozyten: Dichte 1,066 - 1,077 g/ml

Basophile: Dichte 1,075 - 1,081 g/ml

Neutrophile: Dichte 1,080 - 1,099 g/ml

Eosinophile: Dichte 1,088 - 1,096 g/ml

Erythroztyen: Dichte 1,090 - 1,110 g/ml

 

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