IZ medium serum-free, 1 litre

Immune cell medium IZ serum-free

 

Bio&SELL's IZ medium is an optimised serum-free medium for the effective cultivation and propagation of human immune cells (T, NK, CIK and LAK cells) in vitro - "ready-to-use"!
For you, "ready-to-use" means:

    No expensive supplementation (with serum)
    No need for time-consuming serum tests
    No batch to batch variations
    Significantly lower risk of contamination
    Time saving
    Reliable, absolutely reproducible results

 
Composition of the IZ medium:

The basis for the IZ medium is the RPMI 1640 and the DMEM-F12 medium, which have been enriched by the addition of special salts, trace elements, albumin, cholesterol, lipids and vitamins. The IZ medium deliberately does not contain any growth factors (such as recombinant cytokines) for cell differentiation or the enrichment of T cells from whole blood samples. These can thus be added in accordance with individual requirements.

Fields of application:

    Proliferation of T cells from peripheral blood mononuclear cells (PBMC)/whole blood after stimulation.
    Proliferation of NK, LAK and CIK cells after stimulation
    Long-term cultivation of immortalised T cells

 
Cell culture of immune cells

Cells of the peripheral blood are arrested in the G0 phase and must be stimulated with mitogens (plant lectins, e.g. phytohemaggluthinin PHA; or antibodies such as anti-CD3 ) in order to be cultivated for some time in the cell culture.

Tips for stimulating T cells:

    T-cell density of 1x105/ ml
    Add PHA according to the manufacturer's instructions (1-5 μg/ml).
    Incubation time is between 48-72 hrs (depending on the type and origin of the lymphocytes and their use)
    Addition of fresh IZ medium and further stimulation of the T cells is necessary to continue the culture

 

Please note: the proliferation rate of primary lymphocytes from whole blood is limited

Tips for stimulation / cultivation of CIK

CIK are enriched by adding IFN-γ, mAb anti-CD3 (OKT3) and IL-2 to isolated PBMCs (peripheral blood mononuclear cells). After 2-3 weeks in vitro culture, their concentration is increased 1000-fold.

 

Storage temp.: +2°C to +8°C in the dark

Shelf life: 10 months from production date

IZ medium enriched with supplements / growth factors should also be stored at 2 - 8°C in the dark and avoid frequent thawing and freezing, protect from light.