SILAC DMEM - Low Glucose (1.0 g/L) / Without L-Arginine, without L-Lysine, with stab. Glutamine, with phenol red

SILAC DMEM Low Glucose (1.0 g/L) is without L-arginine, without L-lysine, with stable glutamine, with phenol red for labelling with multiple isotope amino acids.

SILAC: Stable isotope labelling with aminoacids in cell culture

It is used to study various aspects, such as protein expression, protein quantification and protein stability,
which are difficult to detect with simple mass spectrometry, by incorporating non-radioactive stable amino acid isotopes into newly synthesised proteins.

SILAC labelling is achieved via normal metabolic processes (e.g. cell division) by incorporating radioactive stable amino acid isotopes into newly synthesised proteins.
amino acid isotopes are incorporated into newly synthesised proteins (e.g. 2H, 13C and 15N atoms).

Due to the accuracy of quantification and the simplicity of interpretation of MS results, the SILAC method offers unique advantages for quantitative and functional proteomics.

SILAC DMEM is formulated without L-arginine and L-lysine for labelling with multiple isotopic amino acids and has no effect on the
morphology or growth rates.