Low Melt Agarose, 250 g

Low Melt Agarose

Bio&SELL Low Melt Agarose (or low meting point agarose) is an agarose with a comparatively low melting point and is ideal for separating fragments between 80 bp and 25,000 bp.

    The melting point is guaranteed to be < 66°C, which is lower than that of universal agarose.
    Excellent for preparative gel electrophoresis
    The cut gel piece with the desired DNA fragment can be melted at a lower temperature and the DNA can thus be obtained more gently
    For the isolation of DNA fragments we recommend the Bio&SELL Double Pure Combi Kit.

Like all our agaroses, each batch of Bio&SELL low melting point agarose undergoes strict quality controls with regard to all relevant parameters.

    Gel strength (1.0%): > 200 g/cm2
    Gelling temperature (1.5%): < 31 °C
    Melting temperature (1.5%): < 66 °C
    Electroendosmosis (-mr): 0.05 - 0.14
    Sulphate content: < 0.10 %
    Water content: < 10.0 %
    DNA binding: not detectable
    DNAse- and RNAse-activity: not detectable

 
Preparation of a standard agarose gel:

 

For 100 ml of a 1% agarose gel, prepare 1 g agarose in 100 ml electrophoresis buffer. After dissolving the agarose by heating and a cooling phase, you can pour the gel.