5x PCR-Mastermix "ready-to-load", 100 μl

5x PCR Mastermixes "ready-to-load" - super fast, very convenient and reliable.
 The Bio&SELL 5x PCR Mastermix "ready-to-load" is a ready-mixed solution for PCR, ready for subsequent direct application onto an agarose gel.

 
Recommended PCR preparation:
Component Volume Final concentration
Bio&SELL
5x PCR Mastermix 10 µl 1x
Primer 1 x µl 0.4 µM
Primer 2 x µl 0.4 µM
Template DNA x µl 5-100 ng
Fill up water to 50 µl     

Stability:

    Thermostable
    1 month stable at room temperature
    6 months stable at 2°C to 8°C

The mix contains all necessary reagents for the PCR reaction, except template DNA, primer and water. Including blue + yellow dye for direct loading onto an agarose gel.

The 5x PCR Mastermix "ready-to-load" is optimised for PCR reactions with subsequent visualisation of the products by agarose gel electrophoresis. The blue dye migrates in a 1% agarose gel at the same speed as 3.5 - 4.5 kb DNA fragments, the yellow dye at about the same speed as primers (< 35 - 45 bp). The Bio&SELL 5x PCR-Mastermix contains Tween-20. If a detergent-free buffer system is required, please contact us at 09128 / 724 32 32.

The Bio&SELL 5x PCR Mastermix is not recommended for applications with subsequent spectrophotometric measurements (absorbance or fluorescence measurements), as the two dyes may interfere with these applications.