5x QPCR EvaGreen® Rox, 5000 μl, 1250 rxn à 20 µl

- Article number BS76.580.5000
This mix contains 1,250 reactions a' 20 µl.
The 5x QPCR Mixes EvaGreen® (Rox), for EvaGreen®-based QRT-PCR are optimised for real-time applications like quantitative PCR and contain all necessary components for quantitative PCR except template DNA and primer.
EvaGreen® is compatible with all common real-time PCR cyclers - just select the default setting for SYBR® Green or FAM!
Application:
Detection and quantification of DNA and cDNA targets
Profiling of gene expression
Microbial detection
Determination of viral loads
Stability:
Thermostable
1 month stable at room temperature
Stable for 6 months at 2°C to 8°C
With internal reference dye Rox.
Rox is a passive internal reference dye used to normalise the fluorescence reporter signal during QPCR.
The final concentration of the Rox dye in the reaction mixture is 500 nM.
Distribution exclusively with our cooperation partner Bio-Budget Technologies.
EvaGreen® is a registered trademark and licensed for sale by BIOTIUM, Inc, Hayward, CA, USA.
SYBR® is a registered trademark of Molecular Probes, Inc.
1. Pflüger-Müller, B., Oo, J.A., Heering, J. et al. The endocannabinoid anandamide has an anti-inflammatory effect on CCL2 expression in vascular smooth muscle cells. Basic Res Cardiol 115, 34 (2020). https://doi.org/10.1007/s00395-020-0793-3