SCRIPTUM High Precise - One-Step RT-PCR, 100 reactions

SCRIPTUM High Precise is a one-step kit for reverse transcription (RT) of specific RNA templates followed by polymerase chain reaction (PCR). 

SCRIPTUM High Precise is ideal for highly sensitive reverse transcription and high fidelity PCRs with high amplification speed. The kit contains a genetically modified reverse transcriptase with improved thermal stability. The SCRIPTUM reverse transcriptase is highly specific and allows high cDNA yields and improved efficiency for complex, long cDNA fragments. A genetically engineered polymerase with proof-reading activity offers 50x higher accuracy and increased processivity, with 2x shorter elongation times, compared to Taq polymerase. The first-class SCRIPTUM High Precise Enzyme Mix in combination with the optimised SCRIPTUM High Precise Reaction Buffer (with high-purity dNTPs) guarantees excellent amplification results. 

SCRIPTUM High Precise contains all reagents required for RT-PCR (except RNA template and primer). The reagents are mixed in a single reaction tube and the reaction can be performed sequentially without having to open the tube. This guarantees quick and easy performance with a minimum of pipetting steps and a reduced risk of contamination.

Properties

One-Step RT-PCR Kit
Suitable for specific RNA templates
Contains all necessary reagents except RNA template and primer

Good to know

The principle of RT-PCR consists of the synthesis of cDNA by reverse transcription (RT) of an RNA template and subsequent amplification of the cDNA by polymerase chain reaction (PCR).

The first step of RT-PCR consists of converting RNA into complementary DNA (cDNA). This is done by the enzyme reverse transcriptase, which uses the RNA as a template to synthesise the cDNA. Once the cDNA has been synthesised, PCR is carried out to amplify the cDNA. Specific primers are used that bind to the cDNA. New DNA strands are synthesised with the help of a DNA polymerase. RT-PCR is used in gene expression analysis, in the diagnosis of infectious diseases and in basic research.

Different RT-PCR types:

• Quantitative RT-PCR (qRT-PCR): This method enables the quantitative determination of the amount of RNA in a sample. It uses fluorescent dyes or probes to monitor amplification in real time.
• Semi-quantitative RT-PCR: This method allows an approximate quantification of the RNA by analysing the amplification in different cycles, but not in real time.
• Multiplex RT-PCR: Several target genes are amplified in a single reaction, which saves time and material.