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- Article number BS76.515.5000
This mix contains 1,250 reactions a' 20 µl.
The 5x Multiplex QPCR Mixes for probe-based QRT-PCR (No Rox or Rox) are optimised for real-time applications, especially quantitative multiplex PCR, and contain all necessary components for quantitative multiplex PCR except template DNA, primer and probe.
The 5x Multiplex QPCR-Mix Rox contains optimised components, especially MultiPlex relevant buffers as well as Bio&SELL Hot-Start Taq-DNA-Polymerase in a proprietary buffer system, which allows the detection of up to 5-10 targets.
Detection and quantification of DNA and cDNA targets
Profiling of gene expression
Determination of viral loads
Specific suitability for multiplex QPCR reactions
1 month stable at room temperature
Stable for 6 months at 2°C to 8°C
Cycler and probe compatibility
Bio&SELL 5x QPCR Mix (Rox) is compatible with any probe system and real-time cyclers that require Rox as a refernce dye,
e.g. ABI PRISM® 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block).
With internal reference dye Rox.
Rox is a passive, internal reference dye used to normalise the fluorescence reporter signal during QPCR. The final concentration of the Rox dye in the reaction mixture is 500 nM.