BioColl® - mouse, 100 ml

Centrifugation medium made from polysucrose; density: 1.086 g/ml; for the isolation of lymphocytes

Product description

Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used for the isolation of cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.086 g/ml is ideal for the isolation of murine lymphocytes. For optimal pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

Properties

• Density: 1.086 g/ml
• For the isolation of murine lymphocytes
• Aqueous solution of highly cross-linked polysucrose

Good to know

Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or in the production of antibodies.

Storage

Store this product at +2 to RT.

For a complete list of the appropriate density of our BioColl® separation solution for your cell type, see FAQ!

BioColl® is a registered trademark of Bio&SELL GmbH.