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BioColl® - for granulocytes, 100 ml

BioColl® - for granulocytes, 100 ml
€34.90 *
Content: 100 Milliliter

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Delivery time approx. 5 working days

  • BS.L.6153
Centrifugation medium made from polysucrose; density: 1.1 g/ml; for the isolation of human... more
Product information

Centrifugation medium made from polysucrose; density: 1.1 g/ml; for the isolation of human granulocytes

Product description

Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used for the isolation of cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.1 g/ml is ideal for the isolation of human granulocytes. For optimal pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

Properties

  • Density: 1.1 g/ml
  • For the isolation of human granulocytes
  • Aqueous solution of highly cross-linked polysucrose


Good to know

Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or in the production of antibodies.

Storage

Store this product at +2 to RT.

 
For a complete list of the appropriate density of our BioColl® separation solution for your cell type, see FAQ!

BioColl® is a registered trademark of Bio&SELL GmbH.

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Technical data


Category: Centrifugation medium
Density: 1.1 g/ml
Specification: For granulocyte isolation
Form: Liquid (transparent)
Volume: 100 ml
Storage: +2°C to RT
Sterile filtered: Yes
Customs tariff number: 38229000

FAQ


Which densities do my cells/cell particles have?

Platelets: density 1.040 - 1.060 g/ml
Monocytes: density 1.059 - 1.068 g/ml
Lymphocytes: density 1.066 - 1.077 g/ml
Basophils: density 1.075 - 1.081 g/ml
Neutrophils: density 1.080 - 1.099 g/ml
Eosinophils: density 1.088 - 1.096 g/ml
Erythroctyes: density 1.090 - 1.110 g/ml

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