BioColl® separation solution - for granulocytes (density 1.1 g/ml)

€99.90 *

Content: 500 Milliliter

Article number BS.L6155
Delivery time: Delivery time approx. 5 working days

Centrifugation medium made from polysucrose; density: 1.1 g/ml; for the isolation of human... more

Product information

Centrifugation medium made from polysucrose; density: 1.1 g/ml; for the isolation of human granulocytes

Product description

Our BioColl® separation solution contains neutral, highly cross-linked polysucrose with an average molecular weight of 400,000 Da. This hydrophilic polymer can be used to prepare aqueous solutions with different densities (up to about 1.2 g/ml), which are used for the isolation of cells or subcellular particles. The appropriate density of the BioColl® separation solution is selected depending on the particle type. A density of 1.1 g/ml is ideal for the isolation of human granulocytes. For optimal pH and osmolality values, the BioColl® separation solution should be adjusted with an acid (preferably amidotrizoic acid (ATS)) and sodium hydroxide solution.

In order to specifically isolate granulocytes, a double polysucrose gradient is required. For this purpose, BioColl® for lymphocytes (BS.L6115) is layered over BioColl® for granulocytes (BS.L6155). Lymphocytes, thrombocytes and monocytes do not penetrate the Biocoll® layer for lymphocytes (density 1.077 g/ml) due to their lower density and can therefore be easily removed. Due to their higher density, granulocytes pass through the BioColl® layer for lymphocytes (density 1.077 g/ml), but not the BioColl® layer for granulocytes (density 1.1 g/ml). After centrifugation, they are located between the two BioColl® layers. The cells can be easily harvested by careful pipetting.

Properties

Density: 1.1 g/ml
For the isolation of human granulocytes
Aqueous solution of highly cross-linked polysucrose

Good to know

Cells (e.g. PBMCs) are isolated by so-called density gradient centrifugation. This physical process is based on the separation of macromolecules according to their sedimentation properties (size, weight) in a density gradient. The isolation of cells or subcellular particles is often the first step in diagnostic investigations, gene expression studies or in the production of antibodies.

Storage

Store this product at +2 to RT.

For a complete list of the appropriate density of our BioColl® separation solution for your cell type, see FAQ!

BioColl® is a registered trademark of Bio&SELL GmbH.

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Publications

Title: CAR NK-92 cell–mediated depletion of residual TCR+ cells for ultrapure allogeneic TCR-deleted CAR T-cell products

Autors: Jonas Kath, Weijie Du, Stefania Martini, Magdi Elsallab, Clemens Franke, Laura Hartmann, Vanessa Drosdek, Viktor Glaser, Maik Stein, Michael Schmueck-Henneresse, Petra Reinke, Hans-Dieter Volk, Mohamed Abou-el-Enein, Dimitrios L. Wagner

Journal, Year: Blood Advances, American Society of Hematology, 2023; 7/15:4124-4134

Title: The Polarity and Specificity of Antiviral T Lymphocyte Responses Determine Susceptibility to SARS-CoV-2 Infection in Patients with Cancer and Healthy Individuals

Autors: Jean-Eudes Fahrner, Imran Lahmar, Anne-Gaëlle Goubet, Yacine Haddad, Agathe Carrier, Marine Mazzenga, Damien Drubay, Carolina Alves Costa Silva, Lyon COVID Study Group, Eric de Sousa, Cassandra Thelemaque, Cléa Melenotte, Agathe Dubuisson, Arthur Geraud, Gladys Ferrere, Roxanne Birebent, Camille Bigenwald, Marion Picard, Luigi Cerbone, Joana R. Lérias, Ariane Laparra, Alice Bernard-Tessier, Benoît Kloeckner, Marianne Gazzano, François-Xavier Danlos, Safae Terrisse, Eugenie Pizzato, Caroline Flament, Pierre Ly, Eric Tartour, Nadine Benhamouda, Lydia Meziani, Abdelhakim Ahmed-Belkacem, Makoto Miyara, Guy Gorochov, Fabrice Barlesi, Alexandre Trubert, Benjamin Ungar, Yeriel Estrada, Caroline Pradon, Emmanuelle Gallois, Fanny Pommeret, Emeline Colomba, Pernelle Lavaud, Marc Deloger, Nathalie Droin, Eric Deutsch, Bertrand Gachot, Jean-Philippe Spano, Mansouria Merad, Florian Scotté, Aurélien Marabelle, Frank Griscelli,
Jean-Yves Blay, Jean-Charles Soria, Miriam Merad, Fabrice André, Juliette Villemonteix, Mathieu F. Chevalier, Sophie Caillat-Zucman, Florence Fenollar, Emma Guttman-Yassky, Odile Launay, Guido Kroemer, Bernard La Scola, Markus Maeurer, Lisa Derosa, Laurence Zitvogel

Journal, Year: Cancer Discovery, American Association of Cancer Research, 2022; 12:958–983

Title: Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients

Autors: M.-N. Theodoraki, S. Laban, E. K. Jackson, R. Lotfi, P. J. Schuler, C. Brunner, T. K. Hoffmann, T. L. Whiteside and L. Hofmann

Journal, Year: British Journal of Cancer, 2021; 125:1677–1686

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Technical data

Category: Centrifugation medium
Density: 1.1 g/ml
Specification: For granulocyte isolation
Form: Liquid (transparent)
Volume: 100 ml
Storage: +2°C to RT
Sterile filtered: Yes
Customs tariff number: 38229000

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FAQ

1. What are the average densities of my cells/cell particles?

Thrombocytes: Density 1.040 - 1.060 g/ml
Monocytes: Density 1.059 - 1.068 g/ml
Lymphocytes: Density 1.066 - 1.077 g/ml
Basophils: Density 1.075 - 1.081 g/ml
Neutrophils: Density 1.080 - 1.099 g/ml
Eosinophils: Density 1.088 - 1.096 g/ml
Erythrocytes: Density 1.090 - 1.110 g/ml

2. Which BioColl separation solution do I need to isolate my cells?

Human lymphocytes: BioColl for lymphocytes - density: 1.077 g/ml (article number: BS.L6115)
Human monocytes: BioColl for monocytes - density: 1.068 + BioColl for lymphocytes - density 1.077 (article numbers: BS.L6815 + BS.L6115)
Human granulocytes: BioColl for lymphocytes - density: 1.077 + BioColl for granulocytes - density 1.1 (article numbers: BS.L6115 + BS.L6155)
Murine lymphocytes: BioColl for murine lymphocytes - density: 1.086 g/ml (article number: BS.L6121)

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Data sheet