BioColl® separating solution density 1.1g/ml, 500 ml
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- Article number BS.L6155
BioColl® separation solution (density 1.100 g/ml, isotonic).
BioColl® separation solutions contain polysucrose, a polymer with a molecular weight of about 400,000 Daltons. This hydrophilic polymer can be used to prepare aqueous solutions up to a density of about 1.2 g/ml. Depending on the target fraction, the appropriate density of the BioColl solution is selected (see list). For optimal values regarding pH and osmolality, BioColl® must be mixed with an acid, preferably amidotrizoic acid (ATS), and caustic soda.
Which density of BioColl® for which cell fraction?
An overview:
Platelets: density 1.040 - 1.060 g/ml
Monocytes: density 1.059 - 1.068 g/ml
Lymphocytes: density 1.066 - 1.077 g/ml
Basophils: density 1.075 - 1.081 g/ml
Neutrophils: density 1.080 - 1.099 g/ml
Eosinophils: density 1.088 - 1.096 g/ml
Erythroctyes: density 1.090 - 1.110 g/ml
BioColl® is a registered trademark of Bio&SELL GmbH.
Storage temp.: +2°C to RT
Autoren: Jonas Kath, Weijie Du, Stefania Martini, Magdi Elsallab, Clemens Franke, Laura Hartmann, Vanessa Drosdek, Viktor Glaser, Maik Stein, Michael Schmueck-Henneresse, Petra Reinke, Hans-Dieter Volk, Mohamed Abou-el-Enein, Dimitrios L. Wagner
Journal, Jahr: Blood Advances, American Society of Hematology, 2023; 7/15:4124-4134
Autoren: Jean-Eudes Fahrner, Imran Lahmar, Anne-Gaëlle Goubet, Yacine Haddad, Agathe Carrier, Marine Mazzenga, Damien Drubay, Carolina Alves Costa Silva, Lyon COVID Study Group, Eric de Sousa, Cassandra Thelemaque, Cléa Melenotte, Agathe Dubuisson, Arthur Geraud, Gladys Ferrere, Roxanne Birebent, Camille Bigenwald, Marion Picard, Luigi Cerbone, Joana R. Lérias, Ariane Laparra, Alice Bernard-Tessier, Benoît Kloeckner, Marianne Gazzano, François-Xavier Danlos, Safae Terrisse, Eugenie Pizzato, Caroline Flament, Pierre Ly, Eric Tartour, Nadine Benhamouda, Lydia Meziani, Abdelhakim Ahmed-Belkacem, Makoto Miyara, Guy Gorochov, Fabrice Barlesi, Alexandre Trubert, Benjamin Ungar, Yeriel Estrada, Caroline Pradon, Emmanuelle Gallois, Fanny Pommeret, Emeline Colomba, Pernelle Lavaud, Marc Deloger, Nathalie Droin, Eric Deutsch, Bertrand Gachot, Jean-Philippe Spano, Mansouria Merad, Florian Scotté, Aurélien Marabelle, Frank Griscelli,
Jean-Yves Blay, Jean-Charles Soria, Miriam Merad, Fabrice André, Juliette Villemonteix, Mathieu F. Chevalier, Sophie Caillat-Zucman, Florence Fenollar, Emma Guttman-Yassky, Odile Launay, Guido Kroemer, Bernard La Scola, Markus Maeurer, Lisa Derosa, Laurence Zitvogel
Journal, Jahr: Cancer Discovery, American Association of Cancer Research, 2022; 12:958–983
Autoren: M.-N. Theodoraki, S. Laban, E. K. Jackson, R. Lotfi, P. J. Schuler, C. Brunner, T. K. Hoffmann, T. L. Whiteside and L. Hofmann
Journal, Jahr: British Journal of Cancer, 2021; 125:1677–1686
Kategorie: Zentrifugationsmedium
Dichte: 1,1 g/ml
Spezifizierung: Zur Erythrozyten-Isolierung
Form: Flüssigkeit (durchsichtig)
Volumen: 500 ml
Lagerung: +2°C bis RT
Steril filtriert: Ja
Zolltarifnummer: 38229000
1. Welche Dichte der BioColl®-Trennlösung brauche ich für welche Zellfraktion?
Thrombozyten: Dichte 1,040 - 1,060 g/ml
Monozyten: Dichte 1,059 - 1,068 g/ml
Lymphozyten: Dichte 1,066 - 1,077 g/ml
Basophile: Dichte 1,075 - 1,081 g/ml
Neutrophile: Dichte 1,080 - 1,099 g/ml
Eosinophile: Dichte 1,088 - 1,096 g/ml
Erythroztyen: Dichte 1,090 - 1,110 g/ml