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HEK-293 medium A - serum-free, 500 ml

HEK-293 medium A - serum-free, 500 ml
€189.00 *
Content: 500 Milliliter

Prices plus VAT plus shipping costs

  • Article number BS.23.525.0500A
  • Delivery time: Delivery time approx. 5 working days
  • Storage temperature: 2 - 8°C
  • Cust. duty tariff no.: 38210000
Description FAQ
Description
HEK-293 Medium  A is a serum-free, ready-to-use medium optimized for the cultivation of... more
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HEK-293 Medium A is a serum-free, ready-to-use medium optimized for the cultivation of adherent HEK-293 cells. The medium is based on DMEM and has been specially enriched with L-glutamine, cholesterol, albumin, vitamins, hormones, growth factors, and additional trace elements (total content of proteins and animal components < 0.2% w/v).

Category Serum-free cell culture media
Product type HEK-293 Medium A
Specification For adherent cells
Form Liquid
Size 500 ml
L-Glutamine Yes
Phenol red Yes
Sterilization Sterile filtered


You can switch from serum-containing medium to our serum-free HEK 293 A medium without any special adaptation. Tips on adaptation can be found in the FAQs.

We would also be happy to send you an adaptation protocol!

 

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FAQ

Adaption von HEK-293-Zellen

Ein Wechsel von serumhaltigem Medium auf unsere HEK-29 Medien ist häufig ohne eine besondere Adaption möglich. Für Zellklone, die ein einfaches Umsetzen in HEK-Medium serumfrei nicht vertragen, empfehlen wir die Zellen in HEK-Medium serumfrei mit Serumzusatz zu kultivieren und den Serumanteil allmählich zu reduzieren. Unterstützt wird dieses Verfahren durch höhere Zelldichten bei der Aussaat.

Für eine erfolgreiche Überführung der Zellen in eine serumfreie Kultur spielt der Vitalitätszustand eine entscheidende Rolle. Daher müssen die Zellen in der logarithmischen Wachstumsphase entnommen werden. Nach unseren Erfahrungen gelingt die Anzucht aus der logarithmischen Phase mit deutlich höheren Erfolgsaussichten.

 

Bei der Umsetzung der Zellen muss sichergestellt werden, dass nach einer eventuellen Ablösung der Zellen mit Trypsin das Enzym durch vollständiges Auswaschen entfernt bzw. durch einen Trypsin-Inhibitor gehemmt wird, da der im Serum vorhandene Neutralisationseffekt wegfällt.

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