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si/ miRNA Kit, 10 reactions

si/ miRNA Kit, 10 reactions
€94.90 *
Content: 10 Reaktionen

Prices plus VAT plus shipping costs

Delivery time approx. 5 working days

  • Article number BS77.411.0010
  • Gefahrgut: Dieser Artikel wird als Gefahrgut versendet. Bitte beachten Sie die erhöhten Versandkosten.
Description FAQ Data sheet
Description
si/ miRNA Kit For isolation of siRNA and miRNA from tissue samples, cells or bacteria. The si/... more
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Product information

si/ miRNA Kit

For isolation of siRNA and miRNA from tissue samples, cells or bacteria.

The si/ miRNA kit can be used to isolate microRNAs (miRNAs, 20-25 nucleotides) and siRNAs (small interfering RNAs) of various origins:

    Gram+ or gram- bacteria
    Tissue samples or biopsies up to 20 mg
    Cell cultures up to 5 x 106 cells
    Tissues embedded in paraffin

 

The kit is enormously fast; the preparation time is 15-30 min. These times are achieved because no DNase digestion is necessary.

In addition, the kit guarantees excellent RNA yields of 100 µg at the highest purity.

 

Features:

    For up to 20 mg tissue
    No DNase digestion necessary
    Preparation time only 15 - 30 minutes
    Binding capacity approx. 100µg RNA
    Yield: depending on type and amount of starting material
    Ratio A260 / A280: 1.7 - 2.0

 

Related links to "si/ miRNA Kit, 10 reactions"
FAQ
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FAQ

Ich habe nach der Extraktion keine RNA detektiert?

 

 

 

Falls Sie nach der Extraktion keine RNA detektieren können, kann es sein, dass die RNA zu gering konzentriert ist. Wir empfehlen Ihnen entweder die Menge des Ausgangsmaterials zu erhöhen oder die RNA nach der Extraktion zu fällen. Fällen Sie die RNA mit einer 30:1-Mischung aus absolutem Ethanol und 3M NaAcetat. Resuspendieren Sie die RNA, nach einem Waschschritt mit 70%-igem Ethanol, in 5-10 µl RNase freiem Wasser. Danach sollte Ihre RNA-Konzentration hoch genug sein um detektiert werden zu können.

 

Data sheet
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Data sheet
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