5x QPCR-Mix (Rox), 200 μl, 50 rxn à 20 µl

This mix contains 200 µl, which corresponds to 50 reactions in a 20 µl preparation!

The 5x QPCR Mixes, for probe-based QRT-PCR (No Rox or Rox) are optimised for real-time applications like quantitative PCR and contain all necessary components for quantitative PCR except template DNA, primer and probe.

 

Bio&SELL 5x QPCR-Mix Rox contains optimised components as well as Bio&SELL Hot-Start Taq DNA polymerase in a proprietary buffer system that also allows the detection of "low copy number targets".

 

Application:

    Detection and quantification of DNA and cDNA targets.
    Profiling of gene expression
    Microbial detection
    Determination of viral loads

Stability:

    Thermostable
    1 month stable at room temperature
    Stable for 6 months at 2°C to 8°C

 

Cycler and probe compatibility

Bio&SELL 5x QPCR Mix (Rox) is compatible with any probe system as well as with real-time cyclers that require a high Rox concentration,

e.g. ABI PRISM® 7000, 7300, 7700, 7900 and 7900HT (including Fast Block).

 

With internal reference dye Rox.

Rox is a passive, internal reference dye used to normalise the fluorescence reporter signal during QPCR. The final concentration of the Rox dye in the reaction mixture is 500 nM.