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BioColl® separasjonsløsning tetthet 1,077 g/ml, med HEPES, 500 ml

BioColl® separasjonsløsning tetthet 1,077 g/ml, med HEPES, 500 ml
€ 84,90
Inhalt: 500 Milliliter

zzgl. MwSt. zzgl. Versandkosten

Leveringstid 2-5 virkedager

  • BS.L.6715
BioColl® separasjonsløsning (tetthet 1,077 g/ml, isotonisk) med 10 mM HEPES. BioColl®... mehr
Produktinformasjon

BioColl® separasjonsløsning (tetthet 1,077 g/ml, isotonisk) med 10 mM HEPES.

BioColl® separasjonsløsninger inneholder polysukrose, en polymer med en molekylvekt på ca. 400 000 dalton. Med denne hydrofile polymeren kan det fremstilles vandige løsninger opp til en tetthet på ca. 1,2 g/ml. Avhengig av målfraksjonen velges passende tetthet for BioColl-løsningen (se liste). For optimale verdier med hensyn til pH og osmolalitet må BioColl® blandes med en syre, fortrinnsvis amidotrizoic acid (ATS), og kaustisk soda.

Hvilken tetthet av BioColl® for hvilken cellefraksjon?
En oversikt:

Blodplater: tetthet 1,040 - 1,060 g/ml.

Monocytter: tetthet 1,059 - 1,068 g/ml

Lymfocytter: tetthet 1,066 - 1,077 g/ml

Basofiler: tetthet 1,075 - 1,081 g/ml

Nøytrofiler: tetthet 1,080 - 1,099 g/ml

Eosinofiler: tetthet 1,088 - 1,096 g/ml

Erytrocytter: tetthet 1,090 - 1,110 g/ml

 

 

BioColl® er et registrert varemerke for Bio&SELL GmbH.

Oppbevaringstemperatur: +2 °C til RT

Weiterführende Links zu "BioColl® separasjonsløsning tetthet 1,077 g/ml, med HEPES, 500 ml"
Publikationen

Titel: CAR NK-92 cell–mediated depletion of residual TCR+ cells for ultrapure allogeneic TCR-deleted CAR T-cell products

Autoren: Jonas Kath, Weijie Du, Stefania Martini, Magdi Elsallab, Clemens Franke, Laura Hartmann, Vanessa Drosdek, Viktor Glaser, Maik Stein, Michael Schmueck-Henneresse, Petra Reinke, Hans-Dieter Volk, Mohamed Abou-el-Enein, Dimitrios L. Wagner

Journal, Jahr: Blood Advances, American Society of Hematology, 2023; 7/15:4124-4134

 

Titel: The Polarity and Specificity of Antiviral T Lymphocyte Responses Determine Susceptibility to SARS-CoV-2 Infection in Patients with Cancer and Healthy Individuals

Autoren: Jean-Eudes Fahrner, Imran Lahmar, Anne-Gaëlle Goubet, Yacine Haddad, Agathe Carrier, Marine Mazzenga, Damien Drubay, Carolina Alves Costa Silva, Lyon COVID Study Group, Eric de Sousa, Cassandra Thelemaque, Cléa Melenotte, Agathe Dubuisson, Arthur Geraud, Gladys Ferrere, Roxanne Birebent, Camille Bigenwald, Marion Picard, Luigi Cerbone, Joana R. Lérias, Ariane Laparra, Alice Bernard-Tessier, Benoît Kloeckner, Marianne Gazzano, François-Xavier Danlos, Safae Terrisse, Eugenie Pizzato, Caroline Flament, Pierre Ly, Eric Tartour, Nadine Benhamouda, Lydia Meziani, Abdelhakim Ahmed-Belkacem, Makoto Miyara, Guy Gorochov, Fabrice Barlesi, Alexandre Trubert, Benjamin Ungar, Yeriel Estrada, Caroline Pradon, Emmanuelle Gallois, Fanny Pommeret, Emeline Colomba, Pernelle Lavaud, Marc Deloger, Nathalie Droin, Eric Deutsch, Bertrand Gachot, Jean-Philippe Spano, Mansouria Merad, Florian Scotté, Aurélien Marabelle, Frank Griscelli,
Jean-Yves Blay, Jean-Charles Soria, Miriam Merad, Fabrice André, Juliette Villemonteix, Mathieu F. Chevalier, Sophie Caillat-Zucman, Florence Fenollar, Emma Guttman-Yassky, Odile Launay, Guido Kroemer, Bernard La Scola, Markus Maeurer, Lisa Derosa, Laurence Zitvogel

Journal, Jahr: Cancer Discovery, American Association of Cancer Research, 2022; 12:958–983

 


Titel:
Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients

Autoren: M.-N. Theodoraki, S. Laban, E. K. Jackson, R. Lotfi, P. J. Schuler, C. Brunner, T. K. Hoffmann, T. L. Whiteside and L. Hofmann

Journal, Jahr: British Journal of Cancer, 2021; 125:1677–1686

 

Tekniske data


Kategorie:
Zentrifugationsmedium

Dichte: 1,077 g/ml

Puffersubstanz: 10 mM HEPES

Spezifizierung: Zur Lymphozyten-Isolierung

Form: Flüssigkeit (durchsichtig)

Volumen: 500 ml

Lagerung: +2°C bis RT

Steril filtriert: Ja

Zolltarifnummer: 38229000

FAQ


1. Welche Dichte der BioColl®-Trennlösung brauche ich für welche Zellfraktion?

Thrombozyten: Dichte 1,040 - 1,060 g/ml

Monozyten: Dichte 1,059 - 1,068 g/ml

Lymphozyten: Dichte 1,066 - 1,077 g/ml

Basophile: Dichte 1,075 - 1,081 g/ml

Neutrophile: Dichte 1,080 - 1,099 g/ml

Eosinophile: Dichte 1,088 - 1,096 g/ml

Erythroztyen: Dichte 1,090 - 1,110 g/ml

 

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